Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/02/13"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
 
==Entry title==
 +
*Redid the restriction/digest on 9/10
 +
*Incubated for 20 mins.
 +
*Added 10 ul of the ladder, and 30 ul of the dna
 +
*Ran gel for more than 30 mins to run gel
 +
*KAH 9 is 687 bp
 +
*KAH 10 is NLS
 +
*Vo120 is 2400 bp for backbone
 +
*Cut out 10B for extraction
 +
*Do another restriction/digest for longer with the enzymes
 
* http://openwetware.org/wiki/Image:2013-02-13_gel.jpg
 
* http://openwetware.org/wiki/Image:2013-02-13_gel.jpg
  

Latest revision as of 21:30, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
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Entry title

  • Redid the restriction/digest on 9/10
  • Incubated for 20 mins.
  • Added 10 ul of the ladder, and 30 ul of the dna
  • Ran gel for more than 30 mins to run gel
  • KAH 9 is 687 bp
  • KAH 10 is NLS
  • Vo120 is 2400 bp for backbone
  • Cut out 10B for extraction
  • Do another restriction/digest for longer with the enzymes
  • http://openwetware.org/wiki/Image:2013-02-13_gel.jpg