Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/02/01"

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==Entry title==
 
==Entry title==
 
* Did restriction/ digest on KAH 9/ KAH 10
 
* Did restriction/ digest on KAH 9/ KAH 10
 +
*Used both tubes KAH9/KAH 10A, KAH 9B, KAH 10B
 
*Incubated for 20 mins. to ensure effectiveness
 
*Incubated for 20 mins. to ensure effectiveness
*Ran gel for 20 mins.  
+
*Ran gel for 20 mins. AT 100V
 +
* 1KBT  9A  10A  9B  10B
 +
*Added 8ul of ladder, 15ul of dna
 
*Gel was unsuccessful, so will have to redo.
 
*Gel was unsuccessful, so will have to redo.
 +
*http://openwetware.org/wiki/Image:2-1-2013_gel.JPG
  
  

Revision as of 10:20, 5 March 2013

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Entry title

  • Did restriction/ digest on KAH 9/ KAH 10
  • Used both tubes KAH9/KAH 10A, KAH 9B, KAH 10B
  • Incubated for 20 mins. to ensure effectiveness
  • Ran gel for 20 mins. AT 100V
  • 1KBT 9A 10A 9B 10B
  • Added 8ul of ladder, 15ul of dna
  • Gel was unsuccessful, so will have to redo.
  • http://openwetware.org/wiki/Image:2-1-2013_gel.JPG