Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/02/01"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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==Entry title==
 
==Entry title==
 
* Did restriction/ digest on KAH 9/ KAH 10
 
* Did restriction/ digest on KAH 9/ KAH 10
 +
*Used both tubes KAH9/KAH 10A, KAH 9B, KAH 10B
 
*Incubated for 20 mins. to ensure effectiveness
 
*Incubated for 20 mins. to ensure effectiveness
*Ran gel for 20 mins.  
+
*Ran gel for 20 mins. AT 100V
 +
* 1KBT  9A  10A  9B  10B
 +
*Added 8ul of ladder, 15ul of dna
 
*Gel was unsuccessful, so will have to redo.
 
*Gel was unsuccessful, so will have to redo.
 +
*http://openwetware.org/wiki/Image:2-1-2013_gel.JPG
  
  

Latest revision as of 21:24, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Entry title

  • Did restriction/ digest on KAH 9/ KAH 10
  • Used both tubes KAH9/KAH 10A, KAH 9B, KAH 10B
  • Incubated for 20 mins. to ensure effectiveness
  • Ran gel for 20 mins. AT 100V
  • 1KBT 9A 10A 9B 10B
  • Added 8ul of ladder, 15ul of dna
  • Gel was unsuccessful, so will have to redo.
  • http://openwetware.org/wiki/Image:2-1-2013_gel.JPG