Haynes Lab:Notebook/Inteins/2013/01/24

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  • Learned about BioBrick cloning
  • Completed a restriction/ digest

---15 microliters of plasmid ---1 microliter of restriction enzyme 1 ---1 microliter of restriction enzyme 2 ---3 microliter of 10x buffer --10 microliters of water

    • check temperature first before running experiment