Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/01/24"

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(Entry title)
(Entry title)
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*Completed a restriction/ digest
 
*Completed a restriction/ digest
  
**15 microliters of plasmid  
+
***15 microliters of plasmid  
 
**1 microliter of restriction enzyme 1
 
**1 microliter of restriction enzyme 1
 
**1 microliter of restriction enzyme 2
 
**1 microliter of restriction enzyme 2

Revision as of 19:39, 3 February 2013

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Entry title

  • Learned about BioBrick cloning
  • Completed a restriction/ digest
      • 15 microliters of plasmid
    • 1 microliter of restriction enzyme 1
    • 1 microliter of restriction enzyme 2
    • 3 microliter of 10x buffer
    • 10 microliters of water
    • check temperature first before running experiment