Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/01/24"

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*Completed a restriction/ digest
 
*Completed a restriction/ digest
  
***15 microliters of plasmid  
+
**15 microliters of plasmid  
 
**1 microliter of restriction enzyme 1
 
**1 microliter of restriction enzyme 1
 
**1 microliter of restriction enzyme 2
 
**1 microliter of restriction enzyme 2
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**10 microliters of water
 
**10 microliters of water
  
**check temperature first before running experiment
+
*check temperature first before running experiment
 +
http://openwetware.org/wiki/Image:2013-01-24_gel.jpg
  
 
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Revision as of 10:55, 1 March 2013

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Entry title

  • Learned about BioBrick cloning
  • Completed a restriction/ digest
    • 15 microliters of plasmid
    • 1 microliter of restriction enzyme 1
    • 1 microliter of restriction enzyme 2
    • 3 microliter of 10x buffer
    • 10 microliters of water
  • check temperature first before running experiment

http://openwetware.org/wiki/Image:2013-01-24_gel.jpg