Haynes Lab:Notebook/Inteins/2013/01/24: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(3 intermediate revisions by the same user not shown)
Line 11: Line 11:
*Completed a restriction/ digest
*Completed a restriction/ digest


---15 microliters of plasmid  
**15 microliters of plasmid  
---1 microliter of restriction enzyme 1
**1 microliter of restriction enzyme 1
---1 microliter of restriction enzyme 2
**1 microliter of restriction enzyme 2
---3 microliter of 10x buffer
**3 microliter of 10x buffer
--10 microliters of water
**10 microliters of water


**check temperature first before running experiment
*check temperature first before running experiment
http://openwetware.org/wiki/Image:2013-01-24_gel.jpg


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 11:55, 1 March 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  • Learned about BioBrick cloning
  • Completed a restriction/ digest
    • 15 microliters of plasmid
    • 1 microliter of restriction enzyme 1
    • 1 microliter of restriction enzyme 2
    • 3 microliter of 10x buffer
    • 10 microliters of water
  • check temperature first before running experiment

http://openwetware.org/wiki/Image:2013-01-24_gel.jpg


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Inteins/2013/01/24&oldid=680174"