Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/01/24"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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*Completed a restriction/ digest
 
*Completed a restriction/ digest
  
---15 microliters of plasmid  
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**15 microliters of plasmid  
---1 microliter of restriction enzyme 1
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**1 microliter of restriction enzyme 1
---1 microliter of restriction enzyme 2
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**1 microliter of restriction enzyme 2
---3 microliter of 10x buffer
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**3 microliter of 10x buffer
--10 microliters of water
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**10 microliters of water
  
**check temperature first before running experiment
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*check temperature first before running experiment
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http://openwetware.org/wiki/Image:2013-01-24_gel.jpg
  
 
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Latest revision as of 21:24, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Entry title

  • Learned about BioBrick cloning
  • Completed a restriction/ digest
    • 15 microliters of plasmid
    • 1 microliter of restriction enzyme 1
    • 1 microliter of restriction enzyme 2
    • 3 microliter of 10x buffer
    • 10 microliters of water
  • check temperature first before running experiment

http://openwetware.org/wiki/Image:2013-01-24_gel.jpg