Haynes Lab:Notebook/Inteins/2013/01/18

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  • Transform plasmids
  • warm plates for 10- 20 mins.
  • add 9 microliters of water
  • add 1 microliters of plasmid
  • add 20 microliters of bacterial cells- should be on ice
  • add the 30 microliters to plates
  • sit in incubator over night