Difference between revisions of "Haynes Lab:Notebook/Inteins/2013/01/18"

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(Entry title)
(Entry title)
Line 9: Line 9:
 
* Transform plasmids  
 
* Transform plasmids  
  
warm plates for 10- 20 mins.
+
*warm plates for 10- 20 mins.
add 9 microliters of water
+
*add 9 microliters of water
add 1 microliters of plasmid
+
*add 1 microliters of plasmid
add 20 microliters of bacterial cells- should be on ice  
+
*add 20 microliters of bacterial cells- should be on ice  
add the 30 microliters to plates  
+
*add the 30 microliters to plates  
sit in incubator over night  
+
*sit in incubator over night  
  
  

Revision as of 18:19, 3 February 2013

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Entry title

  • Transform plasmids
  • warm plates for 10- 20 mins.
  • add 9 microliters of water
  • add 1 microliters of plasmid
  • add 20 microliters of bacterial cells- should be on ice
  • add the 30 microliters to plates
  • sit in incubator over night