Haynes Lab:Notebook/Inteins/2013/01/18: Difference between revisions

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* Transform plasmids  
* Transform plasmids  


warm plates for 10- 20 mins.
*warm plates for 10- 20 mins.
add 9 microliters of water
*add 9 microliters of water
add 1 microliters of plasmid
*add 1 microliters of plasmid
add 20 microliters of bacterial cells- should be on ice  
*add 20 microliters of bacterial cells- should be on ice  
add the 30 microliters to plates  
*add the 30 microliters to plates  
sit in incubator over night  
*sit in incubator over night  





Revision as of 19:19, 3 February 2013

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  • Transform plasmids
  • warm plates for 10- 20 mins.
  • add 9 microliters of water
  • add 1 microliters of plasmid
  • add 20 microliters of bacterial cells- should be on ice
  • add the 30 microliters to plates
  • sit in incubator over night