Haynes Lab:Notebook/Engineering PC-TFs/2014/08/05

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Summary

Picked Colonies for Liquid Cultures
CMV/MV9, BL01, BL05, BL09, RhlI plates had growth while the negative control had no growth.

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ALL PcTF COLONIES WERE PICKED
From left to right, top to bottom:
Experimental plate BL01: One colony.
Experimental plate BL05: Four colonies.
Experimental Plate Bl09: Six colonies.
CMV/MV9 Backbone: Ignore. Did not use the proper plasmid for benchmarking.
Positive control RhlI: Lots of growth. Cells seem good.
Negative control: No growth. Plates seem good.


Grew liquid cultures of the 11 PcTF colonies using 4mL amp resistant LB liquid media.
Put in incubator at 37°C at 238RPM for overnight growth.