Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2014/08/05"

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Negative control: No growth. Plates seem good.
 
Negative control: No growth. Plates seem good.
 
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Grew liquid cultures of the 11 PcTF colonies using 4mL amp resistant LB liquid media.<br>
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Put in incubator at 37°C and 238RPM for overnight growth.
  
 
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Latest revision as of 23:10, 26 September 2017


Hayneslab3.gif
Asu logo 3.gif

Report.pngMain project page
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Summary

Picked Colonies for Liquid Cultures
CMV/MV9, BL01, BL05, BL09, RhlI plates had growth while the negative control had no growth.

Hover name

ALL PcTF COLONIES WERE PICKED
From left to right, top to bottom:
Experimental plate BL01: One colony.
Experimental plate BL05: Four colonies.
Experimental Plate Bl09: Six colonies.
CMV/MV9 Backbone: Ignore. Did not use the proper plasmid for benchmarking.
Positive control RhlI: Lots of growth. Cells seem good.
Negative control: No growth. Plates seem good.


Grew liquid cultures of the 11 PcTF colonies using 4mL amp resistant LB liquid media.
Put in incubator at 37°C and 238RPM for overnight growth.