Haynes Lab:Notebook/Engineering PC-TFs/2013/10/24: Difference between revisions
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'' | <big>''Measure concentration of each fragment''</big>: | ||
''Grow liquid culture of KAH126'' | * Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application | ||
* Go to Nucleic Acid Quantification. | |||
* Pipette 2 µL of each DNA sequence into Take 3 plate. | |||
* Place plate in EPOCH plate reader. | |||
{| {{table}} | |||
| align="center" style="background:#efefef;"| '''Part''' | |||
| align="center" style="background:#efefef;"|'''Concentration''' | |||
|- | |||
| KAH126||143.967 ng/μL | |||
|} | |||
<big>''Cell Culture of KAH126''</big> | |||
'''Grow liquid culture of KAH126''' | |||
*Label 15 ml sterile culture tubes. Fill each tube with <big>'''6 ml'''</big> LB growth medium with Amp. | *Label 15 ml sterile culture tubes. Fill each tube with <big>'''6 ml'''</big> LB growth medium with Amp. | ||
*Use sterile pipette tip to touch bacterial streak and put the tip into LB medium. | *Use sterile pipette tip to touch bacterial streak and put the tip into LB medium. |
Revision as of 14:06, 25 October 2013
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October 24, 2013Sigma-Aldrich Mini-prep Kit
Cell Culture of KAH126 Grow liquid culture of KAH126
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