Haynes Lab:Notebook/Engineering PC-TFs/2013/10/24: Difference between revisions
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==October 24, 2013== | ==October 24, 2013== | ||
<big>''Sigma-Aldrich Mini-prep Kit''</big> | |||
<big>''Harvest cells''</big>: '''Pellet 1–5 ml of an overnight recombinant E. coli culture by centrifugation'''. The optimal volume of culture to use depends upon the plasmid and culture density. For best yields, follow the instructions in the note below. Transfer the appropriate volume of the recombinant E.coli culture to a microcentrifuge tube and pellet cells at 12,000 x g for 1 minute. Discard the supernatant. Note: For best results with recombinant E. coli grown in LB (Luria Broth), use 1–3 ml of culture for high copy plasmids or 1–5 ml of culture for low copy plasmids. | <big>''Harvest cells''</big>: '''Pellet 1–5 ml of an overnight recombinant E. coli culture by centrifugation'''. The optimal volume of culture to use depends upon the plasmid and culture density. For best yields, follow the instructions in the note below. Transfer the appropriate volume of the recombinant E.coli culture to a microcentrifuge tube and pellet cells at 12,000 x g for 1 minute. Discard the supernatant. Note: For best results with recombinant E. coli grown in LB (Luria Broth), use 1–3 ml of culture for high copy plasmids or 1–5 ml of culture for low copy plasmids. | ||
Revision as of 13:56, 25 October 2013
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October 24, 2013Sigma-Aldrich Mini-prep Kit Harvest cells: Pellet 1–5 ml of an overnight recombinant E. coli culture by centrifugation. The optimal volume of culture to use depends upon the plasmid and culture density. For best yields, follow the instructions in the note below. Transfer the appropriate volume of the recombinant E.coli culture to a microcentrifuge tube and pellet cells at 12,000 x g for 1 minute. Discard the supernatant. Note: For best results with recombinant E. coli grown in LB (Luria Broth), use 1–3 ml of culture for high copy plasmids or 1–5 ml of culture for low copy plasmids.
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