Haynes Lab:Notebook/Engineering PC-TFs/2013/10/21: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav) |
|||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==October 21, 2013== | ==October 21, 2013== | ||
<big>''Miniprep liquid cultures''</big> | |||
*Add 100 μL of 7X Lysis Buffer (Blue) to 600 μL of E.coli culture in a 1.5 ml microcentrifuge tube. Mix by inverting tube 4-6times. | *Add 100 μL of 7X Lysis Buffer (Blue) to 600 μL of E.coli culture in a 1.5 ml microcentrifuge tube. Mix by inverting tube 4-6times. | ||
*Add 350 μL of cold Neutralization Buffer (Yellow), mix thoroughly. Neutralization is complete when sample becomes yellow and precipitate has formed. | *Add 350 μL of cold Neutralization Buffer (Yellow), mix thoroughly. Neutralization is complete when sample becomes yellow and precipitate has formed. | ||
Line 17: | Line 17: | ||
Measure concentration of each fragment: | <big>''Measure concentration of each fragment''</big>: | ||
* Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application | * Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application | ||
Line 24: | Line 24: | ||
* Place plate in EPOCH plate reader. | * Place plate in EPOCH plate reader. | ||
{| {{table}} | |||
| align="center" style="background:#efefef;"| '''Part''' | |||
| align="center" style="background:#efefef;"|'''Concentration''' | |||
|- | |||
| KAH126 (1)|| 22.239 ng/μL | |||
|- | |||
| KAH126 (2)||57.672 ng/μL | |||
|- | |||
| CMV+MV9 (1)||11.323 ng/μL | |||
|- | |||
| CMV+MV9 (2)||1.92 ng/μL | |||
|- | |||
| CMV+MV9 (3)||27.394 ng/μL | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Latest revision as of 23:30, 26 September 2017
Main project page Previous entry Next entry | |||||||||||||
October 21, 2013Miniprep liquid cultures
|