Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/10/20"

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(October 20,2013=)
(October 20,2013)
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==October 20,2013==
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==October 20, 2013==
 
''Grow liquid culture of CMV + MV9, KAH126''
 
''Grow liquid culture of CMV + MV9, KAH126''
 
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
 
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.

Revision as of 14:23, 20 October 2013


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October 20, 2013

Grow liquid culture of CMV + MV9, KAH126

  • Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
  • Use sterile pipette tip to touch bacterial streak and put the tip into LB medium.
  • Grow the cultures for 10-12 hrs.

Bacterial Transformation of KAH126 and Ligation of CMV + MV9