Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/10/20"

From OpenWetWare
Jump to: navigation, search
(Summary)
(October 20,2013=)
Line 6: Line 6:
 
| colspan="2"|
 
| colspan="2"|
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
=October 20,2013==
+
==October 20,2013==
 
''Grow liquid culture of CMV + MV9, KAH126''
 
''Grow liquid culture of CMV + MV9, KAH126''
 
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
 
*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.

Revision as of 14:23, 20 October 2013


Hayneslab3.gif
Asu logo 3.gif

<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

October 20,2013

Grow liquid culture of CMV + MV9, KAH126

  • Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
  • Use sterile pipette tip to touch bacterial streak and put the tip into LB medium.
  • Grow the cultures for 10-12 hrs.

Bacterial Transformation of KAH126 and Ligation of CMV + MV9