Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/10/20"

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(Autocreate 2013/10/20 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
(Summary)
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==Summary==
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=October 20,2013==
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''Grow liquid culture of CMV + MV9, KAH126''
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*Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
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*Use sterile pipette tip to touch bacterial streak and put the tip into LB medium.
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*Grow the cultures for 10-12 hrs.
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[[Image:supercali.jpg|200px|Bacterial Transformation of KAH126 and Ligation of CMV + MV9]]
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Revision as of 13:23, 20 October 2013


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October 20,2013=

Grow liquid culture of CMV + MV9, KAH126

  • Label 15 ml sterile culture tubes. Fill each tube with 2 ml LB growth medium with Amp.
  • Use sterile pipette tip to touch bacterial streak and put the tip into LB medium.
  • Grow the cultures for 10-12 hrs.

Bacterial Transformation of KAH126 and Ligation of CMV + MV9