Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17

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September 17, 2013

  • Restriction Digest of CMV promoter
    • for PEG DNA precipitation
Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.