Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17"

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(September 17, 2013)
(September 17, 2013)
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|- valign="top"
 
|- valign="top"
 
| bgcolor=#cfcfcf | '''Reagent'''
 
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Volume'''
+
| bgcolor=#cfcfcf | '''15%'''
| bgcolor=#cfcfcf | '''Reagent'''
+
| bgcolor=#cfcfcf | '''10%'''
| bgcolor=#cfcfcf | '''Volume'''
+
| bgcolor=#cfcfcf | '''7%'''
| bgcolor=#cfcfcf | '''Reagent'''
+
| bgcolor=#cfcfcf | '''5%'''
| bgcolor=#cfcfcf | '''Volume'''
+
| bgcolor=#cfcfcf | '''2%'''
 
|-
 
|-
 
| DNA (plasmid) || 10 μL|| || || ||
 
| DNA (plasmid) || 10 μL|| || || ||

Revision as of 20:08, 21 September 2013


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September 17, 2013

  • Restriction Digest of CMV promoter
    • for PEG DNA precipitation
Reagent 15% 10% 7% 5% 2%
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.