Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17"

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(September 17, 2013)
(September 17, 2013)
Line 18: Line 18:
 
| bgcolor=#cfcfcf | '''Volume'''
 
| bgcolor=#cfcfcf | '''Volume'''
 
|-
 
|-
| DNA (plasmid) || 10 μL|| || ||
+
| DNA (plasmid) || 10 μL|| || || ||
 
|-
 
|-
| XbaI|| 1.0 μL|| || ||
+
| XbaI|| 1.0 μL|| || || ||
 
|-
 
|-
| PstI|| 1.0 μL|| || ||
+
| PstI|| 1.0 μL|| || || ||
 
|-
 
|-
| Green 10x FastDigest buffer || 3.0 μL || || ||
+
| Green 10x FastDigest buffer || 3.0 μL || || || ||
 
|-
 
|-
| dH<sub>2</sub>O || 15 μL || || ||  
+
| dH<sub>2</sub>O || 15 μL || || || ||
 
|-
 
|-
| &nbsp; || 30 μL --> 37°C/ ~10 min.
+
| &nbsp; || 30 μL --> 37°C/ ~10 min. || || || ||
 
|}
 
|}
  

Revision as of 20:02, 21 September 2013


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September 17, 2013

  • Restriction Digest of CMV promoter
    • for PEG DNA precipitation
Reagent Volume Reagent Volume Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.