Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17: Difference between revisions

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{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
|- valign="top"
|- valign="top"
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Volume'''
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Volume'''
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Volume'''
| bgcolor=#cfcfcf | '''Volume'''

Revision as of 21:01, 21 September 2013



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September 17, 2013

  • Restriction Digest of CMV promoter
    • for PEG DNA precipitation
Reagent Volume Reagent Volume Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.


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