Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17"

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(Autocreate 2013/09/17 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
(Summary)
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==Summary==
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==September 17, 2013 ==
 
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{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
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|- valign="top"
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| bgcolor=#cfcfcf | '''Reagent'''
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| bgcolor=#cfcfcf | '''Volume'''
  
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|-
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| DNA (plasmid) || 20.0 μL
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|-
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| SpeI|| 1.0 μL
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|-
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| PstI|| 1.0 μL
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|-
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| Green 10x FastDigest buffer || 3.0 μL
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|-
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| dH<sub>2</sub>O || 5 μL
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|-
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| &nbsp; || 30 μL --> 37°C/ ~10 min.
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|}
  
 
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Revision as of 20:46, 17 September 2013


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September 17, 2013

Reagent Volume
DNA (plasmid) 20.0 μL
SpeI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 5 μL
  30 μL --> 37°C/ ~10 min.