Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17: Difference between revisions
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(Autocreate 2013/09/17 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs) |
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== | ==September 17, 2013 == | ||
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{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | '''Reagent''' | |||
| bgcolor=#cfcfcf | '''Volume''' | |||
|- | |||
| DNA (plasmid) || 20.0 μL | |||
|- | |||
| SpeI|| 1.0 μL | |||
|- | |||
| PstI|| 1.0 μL | |||
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| Green 10x FastDigest buffer || 3.0 μL | |||
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| dH<sub>2</sub>O || 5 μL | |||
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| || 30 μL --> 37°C/ ~10 min. | |||
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Revision as of 20:46, 17 September 2013
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September 17, 2013
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