Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27"

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(Autocreate 2013/03/27 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
(Summary)
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==Summary==
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==3/27/2013==
*  
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{| {{table}}
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| align="center" style="background:#0095B6;"|
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| align="center" style="background:#0095B6;"|'''1'''
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| align="center" style="background:#0095B6;"|'''2'''
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| align="center" style="background:#0095B6;"|'''3'''
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| align="center" style="background:#0095B6;"|'''4'''
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| align="center" style="background:#0095B6;"|'''5'''
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|-
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| DNA Insert||1.4||1.8||2.0||2.5||--
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|-
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| DNA Vector (Kozak)||0.6||0.6||0.6||0.6||0.6
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|-
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| T4 Ligase||1||1||1||1||1
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|-
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| Lign Buffer (2x)||5||5||5||5||5
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|-
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| dH2O||2.0||1.6||1.4||.9||3.7
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|-
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| ||10 µL||10 µL||10 µL||10 µL||10 µL
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|-
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|
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|}
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* In a .5 mL tube, pipette the ligation buffer first.
 +
* Then water, DNA insert, vector, and T4 ligase.
 +
* Once completed, allow them to incubate for 10 minutes.
 +
* Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes.
 +
* Then follow transformation process:
  
  

Revision as of 09:05, 2 April 2013

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3/27/2013

1 2 3 4 5
DNA Insert 1.4 1.8 2.0 2.5 --
DNA Vector (Kozak) 0.6 0.6 0.6 0.6 0.6
T4 Ligase 1 1 1 1 1
Lign Buffer (2x) 5 5 5 5 5
dH2O 2.0 1.6 1.4 .9 3.7
10 µL 10 µL 10 µL 10 µL 10 µL
  • In a .5 mL tube, pipette the ligation buffer first.
  • Then water, DNA insert, vector, and T4 ligase.
  • Once completed, allow them to incubate for 10 minutes.
  • Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes.
  • Then follow transformation process: