Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18: Difference between revisions
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[[Image:gel3_22_2013.jpg|200px|Stock digest 3/18/2013]] | {| {{table}} cellspacing="3" <!-- Digest rxn. table --> | ||
[[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/18/2013]] | |- valign="top" | ||
[http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | |||
| rowspan="7" | [[Image:gel3_22_2013.jpg|200px|Stock digest 3/18/2013]] [[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/18/2013]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
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| DNA (plasmid) || 20.0 μL | |||
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| 10x buffer || 3.0 | |||
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| XbaI || 2.0 | |||
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| dH<sub>2</sub>O || 5.0 | |||
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| || 30 μL --> 37°C/ ~15 min. | |||
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Revision as of 20:23, 22 March 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||
SummaryCutting inserts to be placed in mammalian vector
A) Get biobricks and enzymes from freezer. SpeI, PstI, XbaI The following list of parts are:
Biobrick Restriction Enzymes
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