Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2013/03/05"

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(Summary)
(Summary)
Line 42: Line 42:
 
[[Image:gel3_5_2013_cutout.jpg|500px|thumb|left|Stock digest 3/5/2013]]
 
[[Image:gel3_5_2013_cutout.jpg|500px|thumb|left|Stock digest 3/5/2013]]
  
<size=4>''Transformation of hPCD, fshPCD, flyPCD''
+
<fontsize=4>''Transformation of hPCD, fshPCD, flyPCD''
  
  

Revision as of 19:11, 5 March 2013

Owwnotebook icon.png Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Procedure

A) Get biobricks and enzymes from freezer.

SpeI, PstI, XbaI

The following list of parts are:


  • BL01
  • BL05
  • BL09

B)Follow digest procedure:

  DNA          20.0 µL
  Enzyme 1     1.0  µL
  Enzyme 2     1.0  µL
  10x buffer   3.0  µL
  dH2O         5.0  uL
            
             = 30.0 uL


Digest Order:

Biobrick Restriction Enzymes

  • 1. BL01 XbaI/PstI
  • 2. BL05 XbaI/PstI
  • 3. BL09 XbaI/PstI


Stock digest 3/5/2013
Stock digest 3/5/2013

<fontsize=4>Transformation of hPCD, fshPCD, flyPCD