Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10"

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(Autocreate 2012/12/10 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
(Summary)
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==Summary==
 
==Summary==
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* Gibson PCR
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----
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'''PCR of Gibson BioBricks'''
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* *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions.
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* *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Give me your pSB1A3 so that I can do a retransformation
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Revision as of 10:55, 10 December 2012

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Summary

  • Gibson PCR



PCR of Gibson BioBricks

  • *---Karmella 13:55, 10 December 2012 (EST): Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions.
  • *---Karmella 13:55, 10 December 2012 (EST): Give me your pSB1A3 so that I can do a retransformation