Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10: Difference between revisions
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*When flask is taken out of microwave, make sure that the agarose is completed dissolved. | *When flask is taken out of microwave, make sure that the agarose is completed dissolved. | ||
*Set up wells on gel electrophoresis apparatus. The mold for the first digest is B-10. Use the thin "teeth" and slip in the notches. | *Set up wells on gel electrophoresis apparatus. The mold for the first digest is B-10. Use the thin "teeth" and slip in the notches. | ||
*Add EtBr (ethidium bromide) to the agarose gel. There should be 1 microliter of EtBr per 10ml of agarose gel. Therefore, 6 µL. | * Waited 10-15 minutes for gel to cool. Add EtBr (ethidium bromide) to the agarose gel. There should be 1 microliter of EtBr per 10ml of agarose gel. Therefore, 6 µL. | ||
* | *The gel was poured into tray. Wait twenty minutes for gel to settle. | ||
*Wash the agarose gel flask. | *Wash the agarose gel flask. | ||
*Pipette 15 μL of DNA ladder into first well. Pipette 5μL of PCR reactions into subsequent wells. | *Pipette 15 μL of DNA ladder into first well. Pipette 5μL of PCR reactions into subsequent wells. | ||
*Turn on electrophoresis and let run for thirty minutes. | *Turn on electrophoresis (105 V) and let run for thirty minutes. | ||
[[Image:December_12_2012.jpg|400px|thumb|left|]] | |||
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Revision as of 01:21, 14 December 2012
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Summary
Follow steps for gel electrophoresis.
PCR of Gibson BioBricks
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