Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(fix raw html notebook nav) |
|||
(12 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
|style="background-color: # | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: # | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 23: | Line 23: | ||
*Turn on electrophoresis (105 V) and let run for thirty minutes. | *Turn on electrophoresis (105 V) and let run for thirty minutes. | ||
[[Image:December_12_2012.jpg|400px| | [[Image:December_12_2012.jpg|400px||left|]] | ||
<br><br><br><br><br><br><br><br><br><br> | |||
---- | |||
*Zymo DNA Clean & Concentrator Kit | |||
Follow these steps for Zymo DNA Clean % Concentrator Kit | |||
*Add 30 μL of DNA Binding Buffer to each 15 μL PCR Reaction (#1,2,3,4,5) | |||
*Pipette up and down mixture several times to make sure it's thoroughly mixed. | |||
*Centrifuged at top speed for 30 seconds. Flow through was discarded. | |||
*Added 200 μL of Wash Buffer the column and centrifuged for 30 seconds. Repeated step. | |||
*Spin Column for each PCR rxn placed in new 1.5 mL tube. | |||
*30μL of water was direct added to the column to elute DNA. | |||
[[Image:December_12_2012plateread.jpg|150px||left|]] | |||
<br><br><br><br><br><br><br><br><br><br><br> | |||
---- | ---- |
Latest revision as of 22:19, 26 September 2017
Main project page Previous entry Next entry | |
Summary
Follow steps for gel electrophoresis.
Follow these steps for Zymo DNA Clean % Concentrator Kit
PCR of Gibson BioBricks
|