Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2012/12/07"

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PCR was set with preset settings:
 
PCR was set with preset settings:
  
[[Image:pcrsett.jpg|300px|thumb|]]
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[[Image:pcrsett.jpg|300px]]
  
 
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Revision as of 14:59, 7 December 2012

Owwnotebook icon.png Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Performed another PCR run because lid was not closed entirely yesterday. Next time in lab(Monday), confirmation gel will be run to show that PCR rxns worked.


  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01

# Template Primers
1 hPCD 9, 2
2 Plflex 3, 4
3 BL01 5,10
4 hPCD 9, 7
5 BL01 8,10
μL
Template .5 μL
Primer 1 (10 μm) 1.0 μL
Primer 2 (10 μm) 1.0 μL
2*GoTaq 12. 5
DH2O 10.0 μL

PCR was set with preset settings:

Pcrsett.jpg