Haynes Lab:Notebook/Engineering PC-TFs/2012/12/07
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1. hPCD - Pflex - BL01
PCR was set with preset settings:
There was not much DNA that came from the purification kit. A reason for this is because the bands were smaller than usual, according to Vi. Vi is running another gel digest to cut the backbone from the PSB1A3 vector. Rene said she was also running into problems with minipreping the vectors and cutting it. A follow-up with Dr. Haynes will confirm next step and possible options.