Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06"

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(Summary)
(Summary)
Line 10: Line 10:
 
Dilute primers that came from IDT (10x dilution of concentration)
 
Dilute primers that came from IDT (10x dilution of concentration)
  
BL_Primer09 26.6 nm --> 266 μL dilution
+
*BL_Primer09 26.6 nm --> 266 μL dilution
BL_Primer10 25.7 nm --> 257 μL dilution
+
*BL_Primer10 25.7 nm --> 257 μL dilution
  
 
*Thaw and dilute primers that came  (x10 dilution)
 
*Thaw and dilute primers that came  (x10 dilution)

Revision as of 15:27, 6 December 2012

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Summary

Dilute primers that came from IDT (10x dilution of concentration)

  • BL_Primer09 26.6 nm --> 266 μL dilution
  • BL_Primer10 25.7 nm --> 257 μL dilution
  • Thaw and dilute primers that came (x10 dilution)
  • 90 μL water
  • 10 μL primer stock

(10 μm in each tube for PCR reaction)

  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01