Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06: Difference between revisions

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(Autocreate 2012/12/06 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
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==Summary==
==Summary==
*  
 
Dilute primers that came from IDT (10x dilution of concentration)
 
BL_Primer09 26.6 nm --> 266 μL dilution
BL_Primer10 25.7 nm --> 257 μL dilution
 
*Thaw and dilute primers that came  (x10 dilution)
*90 μL water
*10 μL primer stock
 
(10 μm in each tube for PCR reaction)
 
*Assemblies
 
1. hPCD - Pflex - BL01<br>
2. hPCD - BL01
 





Revision as of 15:27, 6 December 2012

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Summary

Dilute primers that came from IDT (10x dilution of concentration)

BL_Primer09 26.6 nm --> 266 μL dilution BL_Primer10 25.7 nm --> 257 μL dilution

  • Thaw and dilute primers that came (x10 dilution)
  • 90 μL water
  • 10 μL primer stock

(10 μm in each tube for PCR reaction)

  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01