Haynes Lab:Notebook/Engineering PC-TFs/2012/12/06
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Dilute primers that came from IDT (10x dilution of concentration)
(10 μm in each tube for PCR reaction)
1. hPCD - Pflex - BL01
Run PCR with preset GoTaq settings. Need to ask Dr. Haynes to be certain settings are correct for my reactions.
*Digest the pSB1A2/3 plasmid (from Rene) with XbaI & SpeI to get rid of the insert