Haynes Lab:Notebook/Engineering PC-TFs/2012/11/08: Difference between revisions
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==Summary== | ==Summary== | ||
* | Assembly Check of PCR Reactions 1,2,3,4<i> | ||
* Gel Electrophoresis | |||
Follow steps for gel electrophoresis. | |||
*Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | |||
*Create 1% gel by putting .6 grams of agarose into flask. | |||
*Microwave agarose solution for 30 seconds | |||
*Using hot gloves, grab flask out of microwave and swirl flask vigorously. Place back in microwave for 30 more seconds. | |||
*When flask is taken out of microwave, make sure that the agarose is completed dissolved. | |||
*Set up wells on gel electrophoresis apparatus. The mold for the first digest is B-10. Use the thin "teeth" and slip in the notches. | |||
*Add EtBr (ethidium bromide) to the agarose gel. There should be 1 microliter of EtBr per 10ml of agarose gel. Therefore, 6 µL. | |||
*Pour gel into tray. Wait twenty minutes for gel to settle. | |||
*Wash the agarose gel flask. | |||
*Pipette 15 μL of DNA ladder into first well. Pipette 5μL of PCR reactions into subsequent wells. | |||
*Turn on electrophoresis and let run for thirty minutes. | |||
The assembly did not work. No bands were seen when placed under UV light.<b> | |||
Revision as of 17:15, 11 November 2012
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SummaryAssembly Check of PCR Reactions 1,2,3,4
Follow steps for gel electrophoresis.
The assembly did not work. No bands were seen when placed under UV light.
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