Difference between revisions of "Haynes Lab:Notebook/Engineering PC-TFs/2012/11/06"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2012/11/06 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
(Summary)
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==Summary==
 
==Summary==
*  
+
* <u>Gibson Assembly </u>
 +
 
 +
Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.
 +
There is no addition of vector this time.
 +
{|border="1" cellpadding="5" cellspacing="0" align="left"
 +
|-
 +
! scope="col" style="background:#efefef;" |
 +
! scope="col" style="background:#efefef;" | 1
 +
! scope="col" style="background:#efefef;" | 2
 +
 
 +
|-
 +
|Gibson Rxn mix
 +
|15 μL
 +
|15 μL
 +
|-
 +
|DNA fragments
 +
|5 μL
 +
|5 μL
 +
|}
 +
 
 +
<br>
 +
<br>
 +
<br>
  
  

Revision as of 15:23, 6 November 2012

Owwnotebook icon.png Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Gibson Assembly

Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5. There is no addition of vector this time.

1 2
Gibson Rxn mix 15 μL 15 μL
DNA fragments 5 μL 5 μL