Haynes Lab:Notebook/Engineering PC-TFs/2012/10/26: Difference between revisions

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|5 μL
|5 μL
|5 μL
|5 μL
|}
<br>
<br>
{|border="1" cellpadding="5" cellspacing="0" align="left"
|-
! scope="col" style="background:#efefef;" |
! scope="col" style="background:#efefef;" | 1
! scope="col" style="background:#efefef;" | 2
|-
|#1
|1.0 μL
|
|-
|#2
|1.0 μL
|
|-
|#3
|1.0 μL
|
|-
|#4
|
|1.0 μL
|-
|#5
|
|1.0 μL
|-
|Vector
|1.0 μL
|1.0 μL
|-
|dH2O
|1.0 μL
|2.0 μL
|-
|Total
|5.0 μL
|5.0 μL
|}
|}


Revision as of 13:31, 30 October 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

Assembly # Concentration
1 24.41 ng/μL
2 17.025 ng/μL
3 42.325 ng/μL
4 16.706 ng/μL
5 45.015 ng/μL











  • Gibson Assembly

Assembly 1 contains PCR rxns 1,2, and 3. Assembly 2 contains PCR rxns 4,5.

1 2
Gibson Rxn mix 15 μL 15 μL
DNA fragments 5 μL 5 μL



1 2
#1 1.0 μL
#2 1.0 μL
#3 1.0 μL
#4 1.0 μL
#5 1.0 μL
Vector 1.0 μL 1.0 μL
dH2O 1.0 μL 2.0 μL
Total 5.0 μL 5.0 μL




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