Haynes Lab:Notebook/Engineering PC-TFs/2012/09/28

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Summary

  • Thaw and dilute primers (x10 dilution)
  • 90 μL water
  • 10 μL primer stock

(10 μm in each tube for PCR reaction)

  • Assemblies

1. hPCD - Pflex - BL01
2. hPCD - BL01


# Primer
1 BBP-hPCD fwd
2 hPCD - Plflex rev
3 hPCD-Plflex fwd
4 Plfex-BL01 rev
5 Plflex-BL01 fwd
6 BL01-BBS rev
7 hPCD-BL01 rev
8 hPCD-BL01 fwd
















# Template Primers
1 hPCD 1, 2
2 Plflex 3, 4
3 BL01 5,6
4 hPCD 1, 7
5 BL01 8,6
μL
Template .5 μL
Primer 1 (10 μm) 1.0 μL
Primer 2 (10 μm) 1.0 μL
2*GoTaq 12. 5
DH2O 10.0 μL