Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/Entry Base: Difference between revisions
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====Results==== | ====Results==== | ||
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1.17 μg/μL. Need between 20-80 μg/μL. <br> | *1.17 μg/μL. Need between 20-80 μg/μL. <br> | ||
0.5 260/280. Need about 1.8 260/280. Shows major contamination. <br> | *0.5 260/280. Need about 1.8 260/280. Shows major contamination. <br> | ||
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====Causes of Errors==== | ====Causes of Errors==== | ||
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Revision as of 12:51, 30 June 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
Tuesday, June 25thSteps for TransformationUsing KAH06 for Plasmid.
Friday, June 28thMiniprepAims: Run plasmids through miniprep in order to isolate plasmid DNA and determine concentration. Protocol: A. Pre-Miniprep1. Add 3 mL LB into 15 mL tube B. Miniprep1. Pipette 1.5 mL of sample into 2 mL tube. Spin for 3 min @ max setting (13.1g). Repeat once more to spin entire sample. C. DNA Concentration- Take 3 Plate1. Open program Gen 5 2.0 before machine. Results
Causes of Errors
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