Difference between revisions of "Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/Entry Base"

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(Friday, June 28th)
(Tuesday, June 25th)
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Take 1 μL of KAH06.<br>
 
Take 1 μL of KAH06.<br>
 
Add 9 μL of ddH20. <br>
 
Add 9 μL of ddH20. <br>
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 +
 +
----
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==Friday, June 28th==
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====Miniprep====
 +
 +
----
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Aims: Run plasmids through miniprep in order to isolate plasmid DNA and determine concentration
 +
 +
Protocol: <br>
 +
[[Image:Lab01.jpg|200px|]]
  
 
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Revision as of 11:24, 30 June 2013

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Tuesday, June 25th

Steps for Transformation


Using KAH06 for Plasmid.
Take 1 μL of KAH06.
Add 9 μL of ddH20.



Friday, June 28th

Miniprep


Aims: Run plasmids through miniprep in order to isolate plasmid DNA and determine concentration

Protocol:
Lab01.jpg