Difference between revisions of "Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27"

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(Reaction List)
(Reaction List)
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*Run 3 Each
*45 Reactions Run
*Using Variation 2
Variation 2<br> [[Image:Haynes_UPL_fig2.png|300px|Figure 1]

Revision as of 23:01, 27 October 2013

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  • This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.

Reaction List

  Template cDNA Gene Target
Rxn 1: treated cells PDX1
Rxn 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells GAPD (reference gene)
Rxn 6: untreated cells PDX1
Rxn 7: untreated cells MAFA
Rxn 8: untreated cells GLP1R
Rxn 9: untreated cells PCSK1
Rxn 10: untreated cells GAPD (reference gene)
Rxn 11: no template PDX1
Rxn 12: no template MAFA
Rxn 13: no template GLP1R
Rxn 14: no template PCSK1
Rxn 15: no template GAPD (reference gene)
  • Run 3 Each
  • 45 Reactions Run
  • Using Variation 2

Variation 2
[[Image:Haynes_UPL_fig2.png|300px|Figure 1]