Difference between revisions of "Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27"

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(Reaction List)
(Reaction List)
Line 24: Line 24:
 
| Rxn 4: || treated cells || PCSK1
 
| Rxn 4: || treated cells || PCSK1
 
|-
 
|-
| Rxn 5: || treated cells || GAPD (reference gene)
+
| Rxn 5: || treated cells || IAPP
 
|-
 
|-
| Rxn 6: || untreated cells || PDX1
+
| Rxn 6: || treated cells || KCNQ2
 
|-
 
|-
| Rxn 7: || untreated cells || MAFA
+
| Rxn 7: || treated cells || GAPD (reference gene)
 
|-
 
|-
| Rxn 8: || untreated cells || GLP1R
+
| Rxn 8: || untreated cells || PDX1
 
|-
 
|-
| Rxn 9: || untreated cells || PCSK1
+
| Rxn 9: || untreated cells || MAFA
 
|-
 
|-
| Rxn 10: || untreated cells || GAPD (reference gene)
+
| Rxn 10: || untreated cells || GLP1R
 
|-
 
|-
| Rxn 11: || no template || PDX1
+
| Rxn 11: || untreated cells || PCSK1
 
|-
 
|-
| Rxn 12: || no template || MAFA
+
| Rxn 12: || untreated cells || IAPP
 
|-
 
|-
| Rxn 13: || no template || GLP1R
+
| Rxn 13: || untreated cells || KCNQ2
 
|-
 
|-
| Rxn 14: || no template || PCSK1
+
| Rxn 14: || untreated cells || GAPD (reference gene)
 
|-
 
|-
| Rxn 15: || no template || GAPD (reference gene)
+
| Rxn 15: || no template || PDX1
 +
|-
 +
| Rxn 16: || no template || MAFA
 +
|-
 +
| Rxn 17: || no template || GLP1R
 +
|-
 +
| Rxn 18: || no template || PCSK1
 +
|-
 +
| Rxn 19: || no template || IAPP
 +
|-
 +
| Rxn 20: || no template || KCN2Q
 +
|-
 +
| Rxn 21: || no template || GAPD (reference gene)
 
|}
 
|}
  

Revision as of 23:06, 27 October 2013

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UPLassay

  • This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.

Reaction List

REACTION LIST
  Template cDNA Gene Target
Rxn 1: treated cells PDX1
Rxn 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells IAPP
Rxn 6: treated cells KCNQ2
Rxn 7: treated cells GAPD (reference gene)
Rxn 8: untreated cells PDX1
Rxn 9: untreated cells MAFA
Rxn 10: untreated cells GLP1R
Rxn 11: untreated cells PCSK1
Rxn 12: untreated cells IAPP
Rxn 13: untreated cells KCNQ2
Rxn 14: untreated cells GAPD (reference gene)
Rxn 15: no template PDX1
Rxn 16: no template MAFA
Rxn 17: no template GLP1R
Rxn 18: no template PCSK1
Rxn 19: no template IAPP
Rxn 20: no template KCN2Q
Rxn 21: no template GAPD (reference gene)
  • Run 3 Each
  • 45 Reactions Run
  • Using Variation 2

Variation 2
Figure 1

  • Using as Reference, discount gene names on left side