Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/29"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2014/05/29 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
(mm/dd/yyyy)
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==mm/dd/yyyy==
 
==mm/dd/yyyy==
* Insert content here...
+
Transformed GG ligations from yesterday.<br>''Theory: Maybe leaving the reaction overnight allows for more backbone colonies. When I did the first 1:10 LuxR ligation, I got no colonies on the backbone plate but when I did it for RhlR and EsaR, the backbone plate had a bunch of colonies.''
 +
<br><br>
 +
Set up 2-50ul PCR reactions to make more GG-ready mCh sender vector. P097 and P130 50AT and 3:30 ext time.
  
  

Revision as of 14:19, 29 May 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yyyy

Transformed GG ligations from yesterday.
Theory: Maybe leaving the reaction overnight allows for more backbone colonies. When I did the first 1:10 LuxR ligation, I got no colonies on the backbone plate but when I did it for RhlR and EsaR, the backbone plate had a bunch of colonies.

Set up 2-50ul PCR reactions to make more GG-ready mCh sender vector. P097 and P130 50AT and 3:30 ext time.