Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/26"

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(Autocreate 2014/05/26 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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Ran colony PCR reactions from Friday (?). Every lane, including the positive control, had a band of about 200 base pairs. There was no smearing. I don't know what went wrong but I at least learned that you shouldn't run 30 PCR reactions without knowing if the primers will work on the positive control.  
  
  

Revision as of 14:38, 29 May 2014

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Ran colony PCR reactions from Friday (?). Every lane, including the positive control, had a band of about 200 base pairs. There was no smearing. I don't know what went wrong but I at least learned that you shouldn't run 30 PCR reactions without knowing if the primers will work on the positive control.