Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/22: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/05/22 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
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==mm/dd/yyyy== | ==mm/dd/yyyy== | ||
PCR clean up of EsaI PCR and mCh Sender Intermediate for golden gate. Forgot to do DpnI digest of mCh (don't need to for EsaI because the PCR template is kan resistance). | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| mCh Sender int gg||0.055||0.031||1.774||55.337 | |||
|- | |||
| EsaI||0.046||0.026||1.776||45.692 | |||
|} | |||
<br><br> | |||
Going to run golden gate anyway. Backbone plate should have the same number of PCR template transformants as any of the ligation plates. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Reaction''' | |||
| align="center" style="background:#f0f0f0;"|'''Rec Bb''' | |||
| align="center" style="background:#f0f0f0;"|'''LuxI''' | |||
| align="center" style="background:#f0f0f0;"|'''BsmBI''' | |||
| align="center" style="background:#f0f0f0;"|'''T4 Ligase''' | |||
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer''' | |||
| align="center" style="background:#f0f0f0;"|'''H2O''' | |||
|- | |||
| Bb ctrl||0.77||0||0.5||1.25||2||15.48 | |||
|- | |||
| RhlI||0.77||3.2||0.5||1.25||2||12.28 | |||
|- | |||
| LasI||0.77||2.8||0.5||1.25||2||12.68 | |||
|- | |||
| EsaI||0.77||1.9||0.5||1.25||2||13.58 | |||
|} | |||
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Revision as of 11:05, 22 May 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||
mm/dd/yyyyPCR clean up of EsaI PCR and mCh Sender Intermediate for golden gate. Forgot to do DpnI digest of mCh (don't need to for EsaI because the PCR template is kan resistance).
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