Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/16: Difference between revisions

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(Autocreate 2014/05/16 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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Cloning results:
<br>
Backbone only control: 27 colonies
<br>
LuxR receiver: some number 10>x>27
<br>
RhlR receiver: 28-30 colonies
<br>
EsaR receiver: 0 colonies
<br><br>
Should try again with vector:inserts 1:3
<br>
Can screen my receivers with sender media. Can blot the colonies or pick and grow in batches. Or just add to original plate.





Revision as of 14:56, 16 May 2014

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mm/dd/yyyy

Cloning results:
Backbone only control: 27 colonies
LuxR receiver: some number 10>x>27
RhlR receiver: 28-30 colonies
EsaR receiver: 0 colonies

Should try again with vector:inserts 1:3
Can screen my receivers with sender media. Can blot the colonies or pick and grow in batches. Or just add to original plate.