Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/16"

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(Autocreate 2014/05/16 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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Cloning results:
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<br>
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Backbone only control: 27 colonies
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<br>
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LuxR receiver: some number 10>x>27
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<br>
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RhlR receiver: 28-30 colonies
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<br>
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EsaR receiver: 0 colonies
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<br><br>
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Should try again with vector:inserts 1:3
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<br>
 +
Can screen my receivers with sender media. Can blot the colonies or pick and grow in batches. Or just add to original plate.
  
  

Revision as of 14:56, 16 May 2014

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mm/dd/yyyy

Cloning results:
Backbone only control: 27 colonies
LuxR receiver: some number 10>x>27
RhlR receiver: 28-30 colonies
EsaR receiver: 0 colonies

Should try again with vector:inserts 1:3
Can screen my receivers with sender media. Can blot the colonies or pick and grow in batches. Or just add to original plate.