Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15"

From OpenWetWare
Jump to: navigation, search
(mm/dd/yyyy)
(mm/dd/yyyy)
Line 27: Line 27:
 
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
 
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
 
| align="center" style="background:#f0f0f0;"|'''H2O'''
 
| align="center" style="background:#f0f0f0;"|'''H2O'''
 +
|-
 +
| Bb ctrl||7.4||0||0||0.5||1.25||2||8.85
 
|-
 
|-
 
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||3.66
 
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||3.66
Line 33: Line 35:
 
|-
 
|-
 
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||1.8
 
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||1.8
|-
 
| Bb ctrl||7.4||0||0||0.5||1.25||2||8.85
 
 
|}
 
|}
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 09:25, 15 May 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yyyy

Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec 56.995 27 7.407407407


Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl 7.4 0 0 0.5 1.25 2 8.85
Lux Rec 7.4 2.94 2.25 0.5 1.25 2 3.66
Rhl Rec 7.4 2.78 1.68 0.5 1.25 2 4.39
Esa Rec 7.4 4.35 2.7 0.5 1.25 2 1.8