Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15"

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{| {{table}}
 
{| {{table}}
 
| align="center" style="background:#f0f0f0;"|'''Reaction'''
 
| align="center" style="background:#f0f0f0;"|'''Reaction'''
| align="center" style="background:#f0f0f0;"|'''Rec bb'''
+
| align="center" style="background:#f0f0f0;"|'''Rec Bb'''
 
| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
 
| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
 
| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
 
| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
 
| align="center" style="background:#f0f0f0;"|'''BsmBI'''
 
| align="center" style="background:#f0f0f0;"|'''BsmBI'''
| align="center" style="background:#f0f0f0;"|'''T4 ligase'''
+
| align="center" style="background:#f0f0f0;"|'''T4 Ligase'''
| align="center" style="background:#f0f0f0;"|'''T4 ligase buffer'''
+
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
 
| align="center" style="background:#f0f0f0;"|'''H2O'''
 
| align="center" style="background:#f0f0f0;"|'''H2O'''
 
|-
 
|-
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||1.54
+
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||3.66
 
|-
 
|-
| Rhl Rec||7.4||2.78||1.68||0.5||1.25||2||2.27
+
| Rhl Rec||7.4||2.78||1.68||0.5||1.25||2||4.39
 
|-
 
|-
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||-0.32
+
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||1.8
 
|-
 
|-
| Bb control||7.4||0||0||0.5||1.25||2||6.73
+
| Bb ctrl||7.4||0||0||0.5||1.25||2||8.85
 
|}
 
|}
 
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Revision as of 09:09, 15 May 2014

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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mm/dd/yyyy

Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec 56.995 27 7.407407407


Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Lux Rec 7.4 2.94 2.25 0.5 1.25 2 3.66
Rhl Rec 7.4 2.78 1.68 0.5 1.25 2 4.39
Esa Rec 7.4 4.35 2.7 0.5 1.25 2 1.8
Bb ctrl 7.4 0 0 0.5 1.25 2 8.85