Difference between revisions of "Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15"

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(mm/dd/yyyy)
(fix raw html notebook nav)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
 
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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| colspan="2"|
 
| colspan="2"|
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| Receiver int vec||56.995||27||7.407407407
 
| Receiver int vec||56.995||27||7.407407407
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|}
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Reaction'''
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| align="center" style="background:#f0f0f0;"|'''Rec Bb'''
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| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
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| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
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| align="center" style="background:#f0f0f0;"|'''BsmBI'''
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| align="center" style="background:#f0f0f0;"|'''T4 Ligase'''
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| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
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| align="center" style="background:#f0f0f0;"|'''H2O'''
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|-
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| Bb ctrl||7.4||0||0||0.5||1.25||2||8.85
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|-
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| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||3.66
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|-
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| Rhl Rec||7.4||2.78||1.68||0.5||1.25||2||4.39
 +
|-
 +
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||1.8
 
|}
 
|}
 
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Latest revision as of 22:58, 26 September 2017

Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

mm/dd/yyyy

Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec 56.995 27 7.407407407


Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl 7.4 0 0 0.5 1.25 2 8.85
Lux Rec 7.4 2.94 2.25 0.5 1.25 2 3.66
Rhl Rec 7.4 2.78 1.68 0.5 1.25 2 4.39
Esa Rec 7.4 4.35 2.7 0.5 1.25 2 1.8